Katokatz is the only microscopybased method that meets the minimal diagnostic criteria for application in the planning, monitoring and evaluation phase of an sth. Currently, the microscopic kato katz kk stool thick smear technique is the most commonly used. View this image or search images by topic on the public health image library phil. The kato katz technique is widely used in subsaharan africa for the diagnosis of schistosoma mansoni and soiltransmitted helminths. Sterlitech, an industry leader in unique micro and submicron filtration products, announced today that its katokatz technique diagnostic kit is now available for immediate use in the field. Our results show that it is capable of diagnosing sth infections with reasonably low proportions of error, particularly with 2 days of stool collection. Template, stainless steel, plastic, or cardboard fig. Diagnostic accuracy of katokatz and flotac for assessing. Comparison of the thick smear and kato katz techniques for diagnosis of intestinal helminth infections. A list of file names included in the cd or diskettes, with a simple description of the content of each file, should be included.
We compared the kato katz thick smear kato katz and mcmaster egg counting mcmaster methods, which are commonly. Kato is an approach to bring the work done on software agents out of academia and into the public arena. Intestinal schistosomiasis and soiltransmitted helminthiasis. Translating preventive chemotherapy prevalence thresholds for schistosoma mansoni from the kato katz technique into the pointofcare circulating cathodic. Pdf diagnostic performance of a single and duplicate kato. The modified kato technique was the method of choice because it was sensitive, simple to perform, and suitable for field use. Assessing stool quantities generated by three specific kato. The cellophane thick smear kato or kato katz method and formalinether concentration fe techniques are two such modalities commonly used for its diagnosis. The prevalence within grade 7 was 70% confirming the high prevalence in this area. The kato technique is now most commonly used for detecting schistosome eggs. The katokatz technique is the most widely used method detecting soiltransmitted helminth eggs in faecal samples. A comparison of the sensitivity and fecal egg counts of.
Author summary currently, in public health, the reduction in the number of eggs excreted in stools after drug administration is used to monitor the efficacy of drugs against parasitic worms. The aim of our study was to compare the performance of three quantitative techniques. View and download powerpoint presentations on kato katz ppt. Helmtec kit kato katz method for diagnosis and control of helminthiasis, 500 tests per kit, each kit containing. However, detailed analyses of quality control, including falsepositive and faecal egg count fec estimates. Several parasitological techniques are used for the diagnosis of. Comparison of sensitivity and specificity of three diagnostic. Pdf the katokatz technique is the most widely used diagnostic method in epidemiologic surveys and drug efficacy trials. Quality control in the diagnosis of trichuris trichiura and. Parasitological techniques pdf who library cataloguing in publication data. Full text is available as a scanned copy of the original print version. Reliable and accurate diagnosis plays a key role in surveillance, prevention and control of schistosomiasis. Comparative cost assessment of the kato katz and flotac techniques for soiltransmitted helminth diagnosis in epidemiological surveys. Our dosefinding study revealed an excellent tolerability profile of oxantel pamoate in children infected with t trichiura.
This month in jove, determining soiltransmitted helminth infection status and physical fitness of schoolaged children. The kato katz thick smear technique is widely used to assess prevalence and intensity in soiltransmitted helminth sth control programmes, but its usefulness in monitoring anthelminthic drug efficacy needs to be validated and compared with other methods. Pdf the present study compared the katokatz thick smear and formol ether sedimentation techniques. Relative contribution of daytoday and intraspecimen. The most widely used approach is the kato katz technique, which is also recommended by the world health organization. Neglected tropical diseases ntds related pages the kato katz technique in tanzania. Transactions of the royal society of tropical medicine and hygiene 106 3. The kato katz technique is the most widely used diagnostic method in epidemiologic surveys and drug efficacy trials pertaining to intestinal schistosomiasis and soiltransmitted helminthiasis. Introduction to diagnostic medical parasitology katokatz. Determining soiltransmitted helminth infection status and. Aug 14, 2010 the kato katz technique is widely used for the diagnosis of soiltransmitted helminthiasis in epidemiological surveys and is believed to be an inexpensive method. However, the sensitivity of the technique is low, particularly for the detection of lightintensity helminth infections. The flotac technique shows a higher sensitivity for the diagnosis of lightintensity soiltransmitted helminth infections but is reported to be more complex and expensive.
Two malacological surveys were also conducted in the same years in order to define the density and infection rate of the intermediate host. Assessing stool quantities generated by three specific. Compared to the kato katz method, the flotac is a more complicated technique, and hence requires better equipped laboratories 1820 and more extensive training of laboratory workers. Table 1 shows that the total number of helminthic infections was 40. Stool samples were processed only by kato katz technique in the previous study. The sensitivity of the kato katz technique for different infection intensities was calculated using eq 4 and is plotted in fig 3. Indirect measure of worm burden in soiltransmitted helminthiases. Katokatz technique cellophane faecal thick smear pdf, 123kb. It is recognized that the technique has its limits. A recent schistosomiasis prevalence survey of all brazilian states, in which 220,000 fecal samples from students aged 714 years were tested by analyzing two slides using the kato katz technique, revealed an estimated prevalence of more than one million people infected with schistosomiasis throughout the country. The kato katz technique was the most sensitive, due to the higher quantity of stool examined sensitivity threshold 24 epg compared with the mcmaster technique sensitivity threshold 50 epg. The kato katz technique and auramine stain did not recover any other protozoan species, which were all partly recovered by trichrome stain, direct smear and fec methods. It facilitates the detection of parasite eggs that infected subjects pass in their feces. Nov 16, 2016 1967 shelby gt500 barn find and appraisal that buyer uses to pay widow price revealed duration.
It has in the past been used for other helminth eggs as well. Ntds impact more than a billion people globallyonesixth of the worlds population. Templates of different sizes have been produced in different countries. Where the prevalence of sth is download fulltext pdf. However, new tests have been developed in order to facilitate diagnosis, e. Video articles in jove about kato katz technique include august 2012. Fecal examination is the standard diagnostic method to diagnose most parasitic infections of the gastrointestinal tract, which include clonorchis sinensis infection. What is the global impact of neglected tropical diseases. The kato katz technique is recommended for the diagnosis of helminth infections in epidemiological surveys, drug efficacy studies and monitoring of control interventions. For example, due to its low sensitivity at low prevalence levels, a kato katz prevalence measure is likely to be lower than a point. Nine hundred kato katz thick smear preparations were done. An optimum therapeutic dose range of 1530 mgkg oxantel pamoate was defined. May 30, 2019 the kato katz technique is recommended worldwide for the diagnosis of intestinal schistosomiasis, detecting parasite eggs in feces of infected people.
The unreliability of the kato katz technique limits its usefulness for evaluating s. The agreement between the results of the flotac basic technique and duplicate kato katz thick smear readings examined at baseline and followup was assessed for the diagnosis of a. The present study compared the katokatz thick smear and formol ether sedimentation techniques in the diagnosis of schistosoma mansoni infections. Infection with sth can be readily diagnosed by detection of helminth eggs in stool samples using microscopic techniques. Research open access comparative cost assessment of the. Yet, studies comparing diagnostic methods for the enumeration of eggs in stool are few. Global health newsroom neglected tropical diseases ntds. Usefulness of katokatz and trichrome staining as diagnostic. The actual cost of using the katokatz technique is higher and more variable. Diagnostic performance of a single and duplicate kato katz, miniflotac, fecpakg2 and qpcr for the detection and quantification of soiltransmitted helminths in three. Pdf estimating sensitivity of the katokatz technique for the.
Comparison of the katokatz thick smear and mcmaster egg counting techniques for monitoring drug efficacy against soiltransmitted helminths in schoolchildren on pemba island, tanzania. We compared the accuracy of the kato katz method and etherconcentration technique for the diagnosis of soiltransmitted helminth infections. The katokatz technique is recommended for the diagnosis of helminth infections in epidemiological surveys, drug efficacy studies and monitoring. The data indicates that sensitivity of the methods decreased after administration of drugs, but this is expected as fecs drop after treatment. Nevertheless, we concluded that the kato katz method, when used with the thick smear, would be table 2 results of thick smear and kato katz analysis of 258 stool samples from public school children in salinas da margarida county, bahia. Find powerpoint presentations and slides using the power of, find free presentations research about kato katz ppt.
Crane specification search result for manufacturer. Laboratory, parasitological techniques, reference book. However, it is striking that the kato technique is not routinely used in laboratories of developed countries, not even if schistosomiasis is suspected. Jul 11, 2018 the agreement among katokatz technique and pcrelisa laboratorial platform was considered good k 0. Later multiply by the appropriate number see inletinformation of the kato set to give the number of the eggs per gram faeces. The unreliability of the katokatz technique limits its. More detailed instructions can be found in laboratory manuals and in the who manual on preventive chemotherapy in human helminthiasis. Our kit was developed in response to difficulty among research groups, control programs, and health organizations to readily source the materials needed for this testing.
The kato katz technique how the kato katz technique works in areas with moderate to high transmission rates of sthi. Quality control in the diagnosis of trichuris trichiura and ascaris lumbricoides using the kato katz technique. However, the kato katz method has low sensitivity, particularly in areas characterised by lowintensity infections booth et al. A collection of anecdotes and reflections on language and language learning, it frequently recalls the pragmatism of similar texts by polyglot linguists such as bloomfield outline guide. Field scientists utilize kato katz tests to identify. For hookworm the dependence on infection intensity is weak, e. The bell filtration technique was equally sensitive in detecting light infections, but was more tedious and more complicated to perform than the thick smear technique. Download the neglected tropical diseases fact sheet cdc pdf. Nov 23, 20 soiltransmitted helminth infections are a major public health problem. The kato technique also called the katokatz technique is a laboratory method for preparing human stool samples prior to searching for parasite eggs. Performance of katokatz and sedimentation techniques in the di agnosis of schistosoma. Kato katz technique gastroenterology clinical medicine scribd. The agreement among kato katz technique and pcrelisa laboratorial platform was considered good k 0.
Introduction to diagnostic medical parasitology home. Estimating sensitivity of the katokatz technique for the. However, a range of methods, including direct microscopy, formolether concentration fec, mcmaster, flotac, and miniflotac, are avialable. Comparison of the thick smear and katokatz techniques for. To gather prevalence data, two parasitological census surveys were conducted 2000 and 2010 using the kato katz technique. The influence of sampling effort and the performance of the katokatz technique in diagnosing schistosoma mansoni and hookworm coinfections in rural cote divoire volume 127 issue 6 m. The kit was developed in response to the unanimous difficulty among research. Stool specimens were collected over 5 consecutive days, and 5 eggcounts were made in each specimen by the kato katz technique.
Parasitological techniques pdf parasitological techniques pdf download. A comparison of the sensitivity and fecal egg counts of the. Schistosomiasis transmission and environmental change. Coproparasitological diagnosis is still a challenge in management of helminth. Development of a laboratorial platform for diagnosis of. Comparison of the katokatz method and etherconcentration.
The influence of sampling effort and the performance of. In addition, for field surveys and investigational purposes, the egg output can be quantified by using the kato katz technique 20 to 50 mg of fecal material. Quality control in the diagnosis of trichuris trichiura and ascaris. Background schistosomiasis remains one of the most prevalent parasitic infections in the world and has significant economic and public health consequences, particularly in poor communities. The study was carried out in oran, northern argentina. An accurate diagnosis is important in order to identify individuals and communities in need of intervention, and for monitoring drug efficacy and potential emergence of resistance. Achieving elimination as a public health problem for. Helmtec kit katokatz method for diagnosis and control of helminthiasis, 500 tests per kit, each kit containing. It cannot be used to identify hookworm eggs because. On other hand, the agreement of the laboratorial and commercial pcrelisa platforms results was considered excellent k 0. Albonico, m, sm ame, jozef vercruysse, and bruno levecke. Pdf diagnostic performance of a single and duplicate. Field evaluation of a new antibodybased diagnostic for. Current status of intestinal parasitic infections and.
Assessing stool quantities generated by three specific katokatz. We assessed the comparability of the average amount of faeces generated by three kato katz templates included in test kits from two different providers. Comparative cost assessment of the katokatz and flotac. Efficacy and safety of oxantel pamoate in schoolaged. With a weight independent dose of 500 mg oxantel pamoate 95% of children aged 714 years in subsaharan africa would receive doses of 117320 mgkg. Sterlitech, an industry leader in unique micro and submicron filtration products, announced today that its kato katz technique diagnostic kit is now available for immediate use in the field. Katz technique can also be used to classify subjects as positive or negative.
Estimating the sensitivity and specificity of katokatz. For the diagnosis of common sths, the world health organization who recommends the kato katz technique 7,8, which is a relatively straightforward method for determining the prevalence and intensity of such infections. Who has recommended kato katz technique in areas with moderate to high transmission rates of soil transmitted helminths i. The kato katz technique is still the method of choice for detection of sth infection in studies conducted in rural environments with poor infrastructure.
All of them were detected by kato katz followed by the fec 70% and direct smear 32. Variations in the laboratory technique used might be an important factor here among other unforeseen reasons. Comparison of sensitivity and faecal egg counts of mini. The aim of this study was to evaluate the performance of. Comparison of the katokatz thick smear and mcmaster egg. An accurate diagnosis of soiltransmitted helminthiasis is important for individual patient management, for drug efficacy evaluation and for monitoring control programmes. Accuracy, diagnosis, infection intensity, katokatz technique, schistosomes, soiltransmitted helminths multilingual abstracts please see additional file 1 for translation of the abstract into six official working languages of the united nations. Kato katz technique free download as powerpoint presentation. Kato katz technique cellophane faecal thick smear pdf, 123kb. Study data should be submitted to the ogd in electronic format. A promising alternative is the mcmaster egg counting technique. Estimating sensitivity of the katokatz technique for the diagnosis of. Thick smear kato katz positive negative sensitivity specificity kappa index. The kato katz technique is one acceptable technique.
Kato katz technique is used for qualitative and semiquantitative diagnosis of intestinal helminthic infestations. Comparison of the kato katz thick smear and mcmaster egg counting techniques for monitoring drug efficacy against soiltransmitted helminths in schoolchildren on pemba island, tanzania. The kato technique also called the kato katz technique is a laboratory method for preparing human stool samples prior to searching for parasite eggs. Developers can create agents as easily as they can drupal modules. Additionally, individuals are often affected with more than one parasite or infection. Performance of kato katz and sedimentation techniques in the di agnosis of schistosoma mansoni according to the intens ity of in fecti on. Comparison of the bell technique, a modified kato thick smear. The kato katz technique is the most widely used method detecting soiltransmitted helminth eggs in faecal samples. The influence of sampling effort and the performance of the. Katokatz kits contain a roll of cellophane that is cut into small pieces and soaked in methylene blue.